Distinguishing Methicillin-Resistant and Sensitive Staphylococcus aureus Using Volatile Headspace Metabolites

Beike Jia,Brad Sohnlein,Kristien Mortelmans,Michael J Coggiola,Harald Oser

Sensors Journal, IEEE（2010）

引用 19|浏览7

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摘要

This proof-of-concept study used solid-phase microextraction fibers (SPME) to collect headspace vapors from a methicillin sensitive Staphylococcus aureus (MSSA) and a methicillin-resistant Staphylococcus aureus (MRSA) strain grown in vitro in liquid growth medium. The collected molecules were separated and identified using gas chromatography and mass spectrometry (GC/MS). Preliminary results distinguished these two strains and provide a foundation for a biomarker library that could one day serve as a diagnostic tool for identifying specific bacterial infections.

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关键词

biochemistry,biosensors,chromatography,mass spectroscopic chemical analysis,microorganisms,patient diagnosis,bacterial infection,bacterial strain,biomarker,gas chromatography/mass spectrometry analysis,headspace vapors,methicillin-resistant staphylococcus aureus methicillin sensitive staphylococcus aureus,solid-phase microextraction fibers,volatile headspace metabolites,gas chromatography,sensors,data mining,proof of concept,strain,mass spectrometry,spectroscopy

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