Methylobacterium dankookense sp. nov., isolated from drinking water

A pink-pigmented bacterium, designated SW08-7 T was isolated from the drinking water of a water purifier. Cells were Gram-negative, rod-shaped, strictly aerobic, and non-spore-forming. It grew optimally at 25°C, pH 6∼7. Phylogenese analysis based on 16S rRNA gene sequence showed that strain SW08-7 T belongs to the genus Methylobacterium . The highest 16S rRNA gene sequence similarities were found to Methylobacterium mesophilicum JCM 2829 T (96.9%), Methylobacterium brachiatum B0021 T (96.9%), Methylobacterium phyllosphaerae CBMB27 T (96.6%), Methylobacterium radiotolerans JCM 2831 T (96.6%), and Methylobacterium hispanicum GP34 T (96.5%). DNA-DNA hybridization experiment revealed low-level (28.5%) of DNA-DNA relatedness between strain SW08-7 T and Methylobacterium hispanicum . The genomic DNA G+C content was 68.9 mol% and the major isoprenoid quinone was Q-10. The major cellular fatty acid of strain SW08-7 T was C 18:1 ω 7 c (79.8±2.1%). Results of phylogenetic, phenotypic, and biochemical analyses revealed that strain SW08-7 T could be classified as representing a novel species of genus Methylobacterium , for which the name Methylobacterium dankookense sp. nov. is proposed. The type strain is SW08-7 T (=KCTC 22512 T =DSM 22415 1 ).