Sesquiterpene glycosides from Cosmospora joca.

Five novel sesquiterpene glycosides, namely, cosmosporasides A-E (1-5), were isolated from the ethyl acetate extract of the fermented broth of Cosmospora joca. The structures of 1-5 were elucidated on the basis of spectroscopic data analyses, monosaccharide composition analyses, and monosaccharide chirality analyses. The relative configuration of the sesquiterpene moiety of 3-5 was determined by I-based configuration analyses and supported by NOESY assignments. The inhibitory effects of 1 5 on the nitric oxide (NO) production in lipopolysaccharide (LPS)-activated murine macrophage RAW264.7 cells were evaluated; all except 3 inhibited NO production at 100 mu M. 4 was the most potent with an average maximum inhibition and a median inhibitory concentration value of 71.70 +/- 1.23% and 65.98 +/- 0.53 mu M, respectively.