Polyhydroxyalkanoate (PHA) synthesis by class IV PHA synthases employing Ralstonia eutropha PHB(-)4 as host strain.

Manami Hyakutake,Yuta Saito,Satoshi Tomizawa,Kouhei Mizuno,Takeharu Tsuge

BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY（2011）

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摘要

Class IV polyhydroxyalkanoate (PHA) synthase from Bacillus cereus YB-4 (PhaRC(YB4)) or B. megaterium NBRC15308(T). (PhaRC(Bm),) was expressed in Ralstonia eutropha PHB(-)4 to compare the ability to produce PHA and the substrate specificity of PhaRCs. PhaRC(YB4) produced significant amounts of PHA and had broader substrate specificity than PhaRC(Bm).

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关键词

polyhydroxyalkanoate,substrate specificity,class IV synthase,Bacillus,Ralstonia eutropha

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