Clonality and virulence traits of Escherichia coli associated with haemorrhagic septicaemia in turkeys.

R H Olsen, M S Chadfield,Flemming Scheutz,D Vandekerchove,P De Herdt,H Laevens,Patrick Butaye, G Meulemans,Frank Pasmans,G A Willshaw,H R Smith,S M Scotland,B Rowe, Andrew C Thomas,D G White,Shaohua Zhao,Patrick F Mcdermott,Suzan F Ayers, S A Gaines,S Friedman, D D Wagner, J Meng, D Needle,Margaret B Davis,Chitrita Debroy,E J Threlfall,J L Ferguson,L R Ward, K Tamura, Joel T Dudley,Masatoshi Nei,Saravana Kumar,Malabi M Venkatesan, J M Buysse,Dennis J Kopecko,Halvor Sommerfelt, K H Kalland,P Raj, S L Moseley,M K Bhan,Bjorn Bjorvatn,Marilda Carlos Vidotto, C R Goes, J Taque,Amilcar Tanuri,Diogenes Santiago Santos, R Wooley, Kathy R Spears, J S Brown,Lisa K Nolan,O J Fletcher, Benoit Baudry,Stephen J Savarino,Pablo A Vial,James B Kaper,Myron M Levine,Annie Bree, M Dho, J P Lafont,J P Christensen, J Olsen,Magne Bisgaard,Sima S Bilge,Carla R Clausen, W Lau, C J Bell, D Finlay,Holly J Clarke, M J Taylor,H J Ball,Jesus E Blanco, M Blanco,Azucena Mora,W D Da Silveira,Andreia S Ferreira,Marcelo Brocchi, L M De Hollanda, A F P De Castro,Aureo Tatsumi Yamada,Marcelo Lancellotti,Vera Nisaka Solferini,Eliana Guedes Stehling, F De Pace,Gerson Nakazato, A R Pacheco, J B De Paiva,Vanessa Sperandio,Elaine R Delicato, B G De Brito, L C J Gaziri, D A Emery, K V Nagaraja, D P Shaw,Jeffrey A Newman,Francis Dziva,Mark P Stevens, Gilberto Hintermann, H M Fischer, R Crameri, Robert J Hutter, R H Gregersen,Christa Ewers,Harriet H Christensen, Tom Janssen,Lothar H Wieler,Ariane Liebchen,D R Muller,Lilo Greune, Cristiane Leitzke Buss,Martin A Schmidt, Stefan G Kiessling, H C Philipp, Carl J Schwarz,Petra Preikschat,Matthias Voss, A E Jerse, Jiayong Yu,Ben D Tall,Timothy J Johnson,Kylie E Siek, S J Johnson,Curt Doetkott,Sandra C Rosenberger,Adam L Stell,J R Johnson, K S Kim, L Spanjaard,Yvonne Wannemuehler, F L Macrina, Kendall R Jones, D J Ayers, S M Mccowen,Subhashinie Kariyawasam,R M La Ragione,A R Sayers,Martin J Woodward, Jianfeng Xu, H Lior,Vania F Prado,James P Nataro,Helge Karch, K Wachsmuth, F Oerskov, I Oerskov, S J M Mcpeake, J A Smyth,Melha Mellata,Keith Ameiss,Hua Mo,Roy Curtiss,Maryvonne Dhomoulin,Charles M Dozois,Peter Brown, P Arne,Clarisse Desautels,John M Fairbrother,Jeffrey W Touchman, A Moll,Kenneth N Timmis, Paul A Rochelle, John Fry, M J Day, M J Bale,Stefano Morabito, H R Schmidt,Fabio Minelli, P Marianikurkdjian, Franz Allerberger,Karl A Bettelheim,Alfredo Caprioli, T A De Campos,Marcos Perezlosada,Emily B Browne, Alexander Madsen, Thomas Wirth,Raphael P Viscidi,Keith A Crandall,Kylie E Rodriguezsiek,Catherine W Giddings,Eliora Z Ron, Alfonso Fasano,J E Watson, Brian M Martin,Stefano Guandalini, Patricia Guerry,Jerod A Skyberg

AVIAN PATHOLOGY（2011）

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摘要

Fifty-five clinical isolates of avian pathogenic Escherichia coli (APEC) from seven outbreaks of acute haemorrhagic septicaemia in turkeys were characterized by serotyping, plasmid profiling including restriction analysis with HindIII, ribotyping with EcoRI and HindIII, multilocus sequence typing (MLST) and virulence profiling. A clonal relationship was demonstrated for each outbreak according to serotype, plasmid profiling, ribotyping, and MLST. In addition, isolates demonstrated highly similar virulence profiles, as all isolates were positive for F11 pili and possessed genes encoding aerobactin (iucD), increased serum survival (iss), temperature-sensitive haemagglutinin (tsh) and colicin V plasmid operon genes (cva/cvi). However, only 20% of the isolates produced colicin V and 42% exhibited serum resistance. All strains with O group O111 and a single O18ac strain (demonstrating non-clonal DNA profiles) were positive for enteroaggregative heat-stabile toxin (EAST1), while isolates of a single outbreak all possessed the enteroaggregative toxin gene (astA). All isolates were negative for genes encoding verocytotoxins (vtx/stx), iron-repressible protein (irp2), P-fimbria (papC), invasion plasmid antigen (ipaH), attaching and effacing gene (eae), enterohaemolysin (ehxA), and enterotoxins LT, STIa (STp) and STIb (STh). In conclusion, highly similar virulence profiles were demonstrated for isolates of E. coli associated with a single well-defined lesion type of colibacillosis in turkeys; acute haemorrhagic septicaemia. The isolates obtained, however, demonstrated a different phylogenetic background, underlining the importance of using well-defined strain collections for characterization of APEC pathotypes.

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关键词

escherichia coli,multilocus sequence typing,iron

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