Monoclonal antibodies defining mouse tissue antigens encoded by the H-2 region.

A number of mouse hybridomas which secrete monoclonal antibodies (mAb) have been produced in this laboratory, mainly for the purposes of recognizing and studying crossover events within the H-2 complex of recombinant mice. The cross-reaction patterns for these mAb were determined by cytotoxicity tests against a panel of mouse strains carrying H-2 haplotypes a, b, d, f, k, n, p, q, r, s, u, and v, up to fifty-five recombinant H-2 haplotypes, and several H-2 mutant haplotypes. The test results were compared with the latest listing of H-2 haplotypes and genes (Klein et al. 1983) to determine specificity. Numerical specificities are generally not given since B10.W strains were not tested and because many of the specificities are new. The hybridomas were established as described by Ozato and co-workers (1980), with the following modifications: the myeloma line P3X63,Ag8.653 was used as the fusion partner except where noted; RPMI 1640 with 15 % fetal bovine serum, 0.01 mM beta mercaptoethanol and 100 mg/1 garamyacin was used as tissue culture medium; thymocytes were added as feeder cells and fusion procedures were carried out at room temperature. Primary immunizations were by skin grafting and after 3 weeks, the