Characterization of nemotic dental fibroblasts.

Up to now, fibroblasts were known to be im-plied in the production and the maintenance of the extracellular matrix. These cells have also been described in inflammatory proces-ses. But, their activation is at present not des -cribed. Finn researchers have found a new way of fibroblast activation, called nemosis, using 3-dimensional cultures (spheroids). In this study, we cultured spheroids with two fi-broblasts cell types from lung and from den-tal pulp. Then, we followed their growth and observed their cell surface by using electron microscopy. We also focused on the expres-sion of various molecules by using RT-PCR and ELISA test and analysed the cytotoxicity throughout the spheroids.