Knockdown of HMGA1 inhibits human breast cancer cell growth and metastasis in immunodeficient mice.

•Overexpression of HMGA1 has been previously implicated in breast carcinogenesis.•An RNAi-based HMGA1-silencing construct with co-expression of EmGFP was generated.•HMGA1-silenced MDA-MB-231 human breast cancer cells were generated.•Knockdown of HMGA1 impairs anchorage-independent growth and tumorsphere formation.•Knockdown of HMGA1 impairs xenograft growth and metastasis in immunodeficient mice.