Semi-automated solid phase extraction method for the mass spectrometric quantification of 12 specific metabolites of organophosphorus pesticides, synthetic pyrethroids, and select herbicides in human urine.

•Developed and validated a method for the measurement of these analytes in urine.•The method is highly efficient using automation for preparation and analysis.•High mass resolution analysis reduces chemical noise and increases sensitivity.•Smaller diameter, smaller pore size chromatography column improves chromatography.