Transcriptomic analysis of clonal growth rate variation during CHO cell line development.

Padraig Doolan,Colin Clarke,Paula Kinsella,Laura Breen,Paula Meleady,Mark Leonard,Lin Zhang,Martin Clynes,Sinead T Aherne,Niall Barron

Journal of Biotechnology（2013）

引用 27|浏览2

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摘要

•Global transcriptomic analysis of a single CHO cell line development project.•Ten CHO K1 sister clones with similar cell specific productivity varying in terms of growth rates were investigated.•Utilisation of CHO specific microarray to measure mRNA expression levels.•Validation of microarray detected expression changes using qPCR.•Enrichment analysis highlights potential role of miRNA mediated post-transcriptional regulation in CHO growth rate.

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关键词

Chinese hamster ovary,Growth rate,CHO,Microarray,miRNA,Cell line development

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