Loop-mediated isothermal amplification for the rapid detection of Chrysanthemum chlorotic mottle viroid (CChMVd).

•Chrysanthemum chlorotic mottle viroid (CChMVd) causes one of the most serious viral diseases in chrysanthemum in Korea.•A sensitive LAMP assay was developed for rapidly detecting CChMVd infection.•The CChMVd LAMP primer sets were designed using the sequences from nonsymptomatic and symptomatic CChMVd isolates in Korea.•The reaction was carried out at 65°C for 90min, and was improved by adding SYBR Green I dye to the inside of the reaction tube lid prior to amplification.•The results indicate that this LAMP method will be useful for chrysanthemum viroid disease monitoring and detecting CChMVd infectious disease.