Microbial-enzymatic-hybrid biological fuel cell with optimized growth conditions for Shewanella oneidensis DSP-10.

•Hybrid biological fuel cell based on the oxidation of lactate by microorganisms at the anode and oxygen reduction by enzymatic cathode including initial material investigation of the anode.•Shewanella oneidensis DSP-10 anode and Laccase air breathing cathode.•Varying growth conditions of microorganisms for optimized current density and discussion of observed growth condition for biofilm formation.•Silica encapsulation of biomass for device development and study.