Purification of prostatic acid phosphatase (PAP) for structural and functional studies.

PHOSPHATASE MODULATORS(2013)

引用 3|浏览4
暂无评分
摘要
High-scale purification methods are required for several protein studies such as crystallography, mass spectrometry, circular dichroism, and function. Here we describe a purification method for PAP based on anion exchange, L-(+)-tartrate affinity, and gel filtration chromatographies. Acid phosphatase activity and protein concentration were measured for each purification step, and to collect the fractions with the highest acid phosphatase activity the p-nitrophenyl phosphate method was used. The purified protein obtained by the procedure described here was used for the determination of the first reported three-dimensional structure of prostatic acid phosphatase.
更多
查看译文
关键词
Prostatic acid phosphatase,Recombinant protein,High-scale purification,Affinity chromatography,L-(+)-tartrate,Acid phosphatase activity,p-Nitrophenyl phosphate
AI 理解论文
溯源树
样例
生成溯源树,研究论文发展脉络
Chat Paper
正在生成论文摘要