Isolation of a new human scFv antibody recognizing a cell surface binding site to CEACAM1. Large yield production, purification and characterization in E. coli expression system.

•Optimal purification strategy for a high yield production of scFv anti-CEACAM1.•scFv shows a high binding reactivity in ELISA, IF an flow cytometry studies.•scFv is a naturally occurring mixture of monomer and dimer fully characterized.•scFv at 37°C shows the dimer behaves as a reservoir converting slowly into monomer.•scFv at 37°C has a t½ for binding CEACAM1 of 12h as demonstrated by ELISA.