Repair of Nerve Defect with Chitosan Graft Supplemented by Uncultured Characterized Stromal Vascular Fraction in Streptozotocin Induced Diabetic Rats

Regenerative properties of stem cells at the service of nerve repair have been initiated during recent decades. Effects of transplantation of characterized uncultured stromal vascular fraction (SVF) on peripheral nerve regeneration were studied using a rat sciatic nerve transection model. A 10-mm sciatic nerve defect was bridged using a chitosan conduit filled with SVF. In control group, chitosan conduit was filled with phosphate-buffered saline alone. The regenerated nerve fibers were studied 4 weeks, 8 weeks, and 12 weeks after surgery. In sham-operated group, the sciatic nerve was only exposed and manipulated. Behavioral and Functional studies confirmed faster recovery of regenerated axons in SVF transplanted animals than in control group (P < 0.05). Gastrocnemius muscle mass in SVF transplanted animals was found to be significantly more than that in control group. Morphometric indices of the regenerated fibers showed the number and diameter of the myelinated fibers were significantly higher in SVF transplanted animals than in control group. In immunohistochemistry, location of reactions to S-100 in SVF transplanted animals was clearly more positive than that in control group. SVF transplantation combined with chitosan conduit could be considered as a readily accessible source of stromal cells that improve functional recovery of sciatic nerve.