Magnetic Bead Assisted Labeling of Antibodies at Nanogram Scale.
Proteomics(2013)
摘要
There are currently several initiatives that aim to produce binding reagents for proteome‐wide analysis. To enable protein detection, visualization, and target quantification, covalent coupling of reporter molecules to antibodies is essential. However, current labeling protocols recommend considerable amount of antibodies, require antibody purity and are not designed for automation. Given that small amounts of antibodies are often sufficient for downstream analysis, we developed a labeling protocol that combines purification and modification of antibodies at submicrogram quantities. With the support of magnetic microspheres, automated labeling of antibodies in parallel using biotin or fluorescent dyes was achieved.
更多查看译文
关键词
Antibody,Automated protocol,Biotinylation,Labeling,Nanogram scale,Technology
AI 理解论文
溯源树
样例
生成溯源树,研究论文发展脉络
Chat Paper
正在生成论文摘要