A simple and highly efficient method to identify the integration site of a transgene in the animal genome.

Neuroscience Research(2014)

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摘要
•A new and highly efficient protocol of inverse PCR is presented.•We have analyzed integration details of two transgenes, NSE-tTA and tetO-Cre.•An insertion effect was observed on one of the neighboring genes of the transgenes.
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关键词
Transgenic mouse,Inverse PCR,NSE-tTA,tetO-Cre,Insertion effect
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