Development of a multiplex RT-PCR-ELISA to identify four distinct species of tospovirus.

Journal of Virological Methods(2014)

引用 11|浏览4
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摘要
•A multiplex RT-PCR-ELISA for identifying four distinct species of tospovirus was developed.•The developed multiplex RT-PCR-ELISA was 10–1000 times more sensitive for tospovirus identification in field-collected plant samples than conventional RT-PCR.•The developed multiplex RT-PCR-ELISA assay can be applied to other tospovirus species.
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关键词
Multiplex,RT-PCR-ELISA,Tospovirus,Nucleocapsid gene
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