Live visualization and quantification of pathway signaling with dual fluorescent and bioluminescent reporters.
Biochemical and Biophysical Research Communications(2014)
Abstract
•Monitoring the dynamics of signaling pathways in living cells is challenging.•Dual reporters can visualize and quantify pathway signaling in live cells.•We constructed and assessed several signaling pathway dual reporters.•We demonstrated the potential of dual reporters to study pathway signaling.•The system is amenable to single cell analysis and high throughput format.
MoreTranslated text
Key words
CMV,EF1α,FBS,GFP,HEK 293,IL-1β,Insu,ITR,Luc,MAPK,mCMV,NF-κB,PKA,PKC,PMA,Puro,RFP,T2A,TF,TNFα,TRE
AI Read Science
Must-Reading Tree
Example
Generate MRT to find the research sequence of this paper
Chat Paper
Summary is being generated by the instructions you defined