Immunological Profile Of Periapical Endodontic Infections From Hiv- And Hiv Plus Patients

AimTo evaluate CD4(+)CD28(+) and CD8(+) T-cell genes and the gene expression of IFN-, TNF-, IL-1-, IL-17A, IL-10, CCL-2/MCP-1, CCL-4, CCL-5 (RANTES), CXCR4, CCR5 and RANKL from cells in the periapical interstitial fluid from root canal infections in healthy patients (HIV-) and HIV-positive individuals (HIV+).MethodologySubjects included 20 HIV- and 23 HIV+ patients referred to the School of Dentistry at the Universidade Federal de Minas Gerais (Belo Horizonte, MG, Brazil). Almost all HIV+ patients were undergoing highly active antiretroviral therapy (HAART). Clinical samples were taken from teeth with pulp necrosis, and no patients had acute periapical symptoms at the time of the appointments. After cleaning and drying, 3 paper points were introduced into the root canal, passing passively through the root apex (2mm) into the periapical tissues for 1min. The samples were collected immediately after root canal cleaning and 7days later (restrained root canal bacterial load) to characterize those gene expressions using real-time PCR.ResultsSignificantly higher levels of CD4(+)CD28(+) and CD8(+) T cells in teeth with restrained bacterial loads (second collection) compared with the first collection were observed in both HIV- and HIV+ samples. In HIV- patients, an increase in IL-10 and CXCR4 expression was demonstrated as well as a decrease in pro-inflammatory cytokines such as RANKL, IFN-, IL1- and CCL5. However, in HIV+ patients an increase in cytokines IFN-, IL-1-, TNF- and IL-17A, and chemokines CCL-2, CXCR4 and CCR5 were observed. The chemokine CCL-5 was not detected in HIV+ individuals.ConclusionsThese findings suggest that after reducing the root canal bacterial load in HIV- individuals an anti-inflammatory response is generated whilst in HIV+ patients a pro-inflammatory response is sustained in the periapical area.