Two-step procedure for evaluating experimentally induced DNA damage: Texas Red and Comet assays.

Gina J Ferris,Lauren B Shunkwiler,Charles A Kunos

CERVICAL CANCER: METHODS AND PROTOCOLS（2015）

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摘要

The technique of Texas Red immunostaining for cellular γH2AX identifies by fluorescence microscopy DNA undergoing active remodeling or repair. To fully characterize γH2AX foci, the technique of alkaline single cell electrophoresis (Comet) assay quantifiably resolves DNA double-strand breaks from other types of DNA damage. When used together, these two techniques may provide evidence for radiochemotherapy-induced DNA damage.

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关键词

Radiation damage,DNA repair,Ribonucleotide reductase,Salvage pathway,gamma H2AX foci,Texas red,Comet,Olive tail moment

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