RApid Parallel Protein EvaluatoR (RAPPER), from gene to enzyme function in one day.
CHEMICAL COMMUNICATIONS(2015)
Abstract
Cell-free transcription-translation systems offer an effective and versatile platform to explore the impact of genetic variations on protein function. We have developed a protocol for preparing linear, mutagenic DNA templates for direct use in the PURE system, enabling the fast and semi-quantitative evaluation of amino acid variations on catalytic activity and stereo-selectivity in native and engineered variants of Old Yellow Enzyme.
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Key words
protein,enzyme,rapper,gene
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