Cu2+-assisted two dimensional charge-mass double focusing gel electrophoresis and mass spectrometric analysis of histone variants.
Analytica Chimica Acta(2014)
摘要
•Chelation of Cu2+ with histones changes migration patterns in gel electrophoresis.•Histone variants were first separated by charge differences.•Charge-separated histone variants were further separated by their mass differences.•Two dimensional separations significantly resolve histone variants.
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关键词
Histones,Gel electrophoresis,Mass spectrometry,Cu2+ chelation
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