Validation of a lipoprotein(a) particle concentration assay by quantitative lipoprotein immunofixation electrophoresis.

•Lp(a) particle concentration [Lp(a)-P] determined by immunofixation electrophoresis.•This Lp(a)-P method (Lipo-IFE) is not influenced by apolipoprotein(a) isoform size.•Lipo-IFE is a simple method, suitable for routine use in the clinical laboratory.•Whether Lipo-IFE will outperform Lp(a) mass assays in assessing CV risk is unknown.