Long term perfusion system supporting adipogenesis.

Rosalyn D Abbott,Waseem K Raja,Rebecca Y Wang,Jordan A Stinson,Dean L Glettig,Kelly A Burke,David L Kaplan

Methods（2015）

引用 36|浏览25

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摘要

•2D and 3D perfusion culture techniques supported adipogenesis of preadipocytes.•3D perfusion contained adipocytes that were lost in the 2D perfused cultures.•3D perfusion stimulated greater lipogenesis and lipolysis compared to 2D perfusion.•3D perfusion stimulated decreased amounts of LDH compared to 2D perfusion.•Greater glycerol was secreted with perfusion versus static cultures.

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关键词

Adipose tissue engineering,Three dimensional culture,Silk scaffold,Long term culture,Perfusion

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