Application of dynamic metabolomics to examine in vivo skeletal muscle glucose metabolism in the chronically high-fat fed mouse.

•Dynamic metabolomics was used to investigate muscle glucose metabolism in vivo.•Mitochondrial TCA cycle metabolism is altered in muscle of HFD mice.•This defect was not pyruvate dehydrogenase mediated, as has been previously thought.•Mitochondrial TCA cycle anaplerosis in muscle is virtually absent during the OGTT.