Exploiting the multiplexing capabilities of tandem mass tags for high-throughput estimation of cellular protein abundances by mass spectrometry.
Methods(2015)
摘要
•Global estimation of absolute protein abundances in complex samples with increased throughput compared to existing methods.•Approach applicable to most LC−MS platforms and samples.•Quantification with tandem mass tags achieved very high precision between replicates.•The in-house software tool employed for this study is publicly available.
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关键词
Mass spectrometry,Absolute protein quantification,Tandem mass tags,Schizosaccharomyces pombe,HEK 293,iBAQ
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