In vivo single-cell labeling by confined primed conversion

William P Dempsey, Lada Georgieva,Patrick M Helbling,Ali Y Sonay,Thai V Truong, Michel Haffner,Periklis Pantazis

NATURE METHODS（2015）

引用 57|浏览11

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摘要

Primed conversion of proteins such as Dendra2 confines photoconversion to a limited 3Dvolume through a sequential absorption process relying on illumination with two separate low-intensity lasers and enables precise targeting of single cells.

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关键词

Fluorescence imaging, Multiphoton microscopy

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