Single-Cell Detection and Collection of Persister Bacteria in a Directly Accessible Femtoliter Droplet Array.

Ryota Iino,Shouichi Sakakihara,Yoshimi Matsumoto,Kunihiko Nishino

BACTERIAL PERSISTENCE: METHODS AND PROTOCOLS（2016）

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摘要

A directly accessible femtoliter droplet array as a platform for single-cell detection and collection of persister bacteria is described. Device microfabrication, femtoliter droplet array formation and concomitant enclosure of single cells, long-term culture and observation of single cells in droplets, and collection of identified persisters from single droplets are described in detail.

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关键词

Drug tolerance,Microdevice,Microdroplet array,Microfabrication,Micropipette,Optical microscopy,Persister,Pseudomonas aeruginosa,Single-cell analysis

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