Micro Regional Heterogeneity Of Cu-64-Atsm And F-18-Fdg Uptake In Canine Soft Tissue Sarcomas: Relation To Cell Proliferation, Hypoxia And Glycolysis

ObjectivesTumour microenvironment heterogeneity is believed to play a key role in cancer progression and therapy resistance. However, little is known about micro regional distribution of hypoxia, glycolysis and proliferation in spontaneous solid tumours. The overall aim was simultaneous investigation of micro regional heterogeneity of Cu-64-ATSM (hypoxia) and F-18-FDG (glycolysis) uptake and correlation to endogenous markers of hypoxia, glycolysis, proliferation and angiogenesis to better therapeutically target aggressive tumour regions and prognosticate outcome.MethodsExploiting the different half-lives of Cu-64-ATSM (13h) and F-18-FDG (2h) enabled simultaneous investigation of micro regional distribution of hypoxia and glycolysis in 145 tumour pieces from four spontaneous canine soft tissue sarcomas. Pairwise measurements of radioactivity and gene expression of endogenous markers of hypoxia (HIF-1 alpha, CAIX), glycolysis (HK2, GLUT1 and GLUT3), proliferation (Ki-67) and angiogenesis (VEGFA and TF) were performed. Dual tracer autoradiography was compared with Ki-67 immunohistochemistry.ResultsMicro regional heterogeneity in hypoxia and glycolysis within and between tumour sections of each tumour piece was observed. The spatial distribution of Cu-64-ATSM and F-18-FDG was rather similar within each tumour section as reflected in moderate positive significant correlations between the two tracers (rho = 0.3920-0.7807; p = 0.0180 -<0.0001) based on pixel-to-pixel comparisons of autoradiographies and gamma counting of tumour pieces. Cu-64-ATSM and F-18-FDG correlated positively with gene expression of GLUT1 and GLUT3, but negatively with HIF-1 alpha and CAIX. Significant positive correlations were seen between Ki-67 gene expression and 64Cu-ATSM (rho = 0.5578, p = 0.0004) and F-18-FDG (rho = 0.4629-0.7001, p = 0.0001-0.0151). Ki-67 gene expression more consistently correlated with F-18-FDG than with Cu-64-ATSM.ConclusionsMicro regional heterogeneity of hypoxia and glycolysis was documented in spontaneous canine soft tissue sarcomas. Cu-64-ATSM and F-18-FDG uptakes and distributions showed significant moderate correlations at the micro regional level indicating overlapping, yet different information from the tracers. F-18-FDG better reflected cell proliferation as measured by Ki-67 gene expression than Cu-64-ATSM.