Development of an Intracellular Screen for New Compounds Able to Inhibit Mycobacterium Tuberculosis Growth in Human Macrophages
Antimicrobial agents and chemotherapy(2016)
摘要
Here we describe the development and validation of an intracellular high-throughput screening assay for finding new antituberculosis compounds active in human macrophages. The assay consists of a luciferase-based primary identification assay, followed by a green fluorescent protein-based secondary profiling assay. Standard tuberculosis drugs and 158 previously recognized active antimycobacterial compounds were used to evaluate assay robustness. Data show that the assay developed is a short and valuable tool for the discovery of new antimycobacterial compounds.
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