On-line comprehensive two-dimensional HepG2 cell membrane chromatographic analysis system for charactering anti-hepatoma components from rat serum after oral administration of Radix scutellariae: A strategy for rapid screening active compounds in vivo.

•A comprehensive 2D CMC system was developed and applied to characterize active components from drug-containing serum for the first time.•A matrix interference deduction method effectively eliminated the interference of endogenous substances in serum.•Wogonin and oroxylin A screened both in vitro and in vivo, could be selected as anti-hepatoma leading compounds.•The methods had advantages in simplicity, sensitivity, accurate recognition and identification.