Benzoxazepines Achieve Potent Suppression of IL‐17 Release in Human T‐Helper 17 (TH17) Cells Through an Induced‐Fit Binding Mode to the Nuclear Receptor RORγ

ROR gamma t, an isoform of the retinoic acid-related orphan receptor gamma (RORc, ROR gamma), has been identified as the master regulator of T-helper 17 (T(H)17) cell function and development, making it an attractive target for the treatment of autoimmune diseases. Validation for this target comes from antibodies targeting interleukin-17 (IL-17), the signature cytokine produced by T(H)17 cells, which have shown impressive results in clinical trials. Through focused screening of our compound collection, we identified a series of N-sulfonylated benzoxazepines, which displayed micromolar affinity for the ROR gamma ligand-binding domain (LBD) in a radioligand binding assay. Optimization of these initial hits resulted in potent binders, which dose-dependently decreased the ability of the ROR gamma-LBD to interact with a peptide derived from steroid receptor coactivator 1, and inhibited the release of IL-17 secretion from isolated and cultured human T(H)17 cells with nanomolar potency. A cocrystal structure of inverse agonist 15 (2-chloro-6-fluoro-N-(4-{[3-(trifluoromethyl) phenyl] sulfonyl}-2,3,4,5-tetrahydro-1,4-benzoxazepin-7-yl) benzamide) bound to the ROR gamma-LBD illustrated that both hydrophobic interactions, leading to an induced fit around the substituted benzamide moiety of 15, as well as a hydrogen bond from the amide NH to His479 seemed to be important for the mechanism of action. This structure is compared with the structure of agonist 25 (N-(2-fluorophenyl)-4[(4-fluorophenyl) sulfonyl]-2,3,4,5-tetrahydro-1,4-benzoxazepin6-amine) and structures of other known ROR gamma modulators.