Hot, humid air decontamination of a C-130 aircraft contaminated with spores of two acrystalliferous Bacillus thuringiensis strains, surrogates for Bacillus anthracis.
JOURNAL OF APPLIED MICROBIOLOGY(2016)
摘要
AimTo develop test methods and evaluate survival of Bacillus thuringiensis kurstaki cry(-) HD-1 and B.thuringiensis Al Hakam spores after exposure to hot, humid air inside of a C-130 aircraft. Methods and ResultsBacillus thuringiensis spores were either pre-inoculated on 1x2 or 2x2cm substrates or aerosolized inside the cargo hold of a C-130 and allowed to dry. Dirty, complex surfaces (10x10cm) swabbed after spore dispersal showed a deposition of 8-10 log(10)m(-2) through the entire cargo hold. After hot, humid air decontamination at 75-80 degrees C, 70-90% relative humidity for 7days, 87 of 98 test swabs covering 098m(2), showed complete spore inactivation. There was a total of 167log(10) live CFU detected in 11 of the test swabs. Spore inactivation in the 98 test swabs was measured at 706log(10)m(-2). ConclusionsLaboratory test methods for hot, humid air decontamination were scaled for a large-scale aircraft field test. The C-130 field test demonstrated that hot, humid air can be successfully used to decontaminate an aircraft. Significance and Impact of the StudyTransition of a new technology from research and development to acquisition at a Technology Readiness Level 7 is unprecedented.
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关键词
aircraft,Bacillus,decontamination,hot humid air,spore,surrogate
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