Toward selective CK2alpha and CK2alpha' inhibitors: Development of a novel whole-cell kinase assay by Autodisplay of catalytic CK2alpha'.

•Surface display of catalytically active human CK2alpha’ via Autodisplay.•Development of a novel selectivity assay for inhibitors of CK2alpha and CK2alpha’.•Screening and comparison of inhibitors toward isoform CK2 holoenzymes.