Simultaneous enantioselective determination of phenylpyrazole insecticide flufiprole and its chiral metabolite in paddy filed ecosystem by ultra-high performance liquid chromatography/tandem mass spectrometry.

•Flufiprole and its chiral metabolite were baseline separated and enantioseparated.•Optical pure stereoisomers were prepared and their elution order were distinguished.•Clean-up/enrichment procedure was based on the modification of QuEChERS method.•Cleanup step was further improved by solid phase extraction (SPE) technology.•Analysis of samples was accomplished by chiral UPLC–MS/MS