Y-chromosomal testing of brown bears (Ursus arctos): Validation of a multiplex PCR-approach for nine STRs suitable for fecal and hair samples

•Validation of a DNA profiling system for brown bear 9 Y-chromosomal STRs•Development of two multiplex PCR reactions suitable for feces, hair and tissue.•The multiplex PCR reactions were amplified down to 0.2ng template input.•Have applications for resolving paternity and family structures.•Provide Y-haplotypes useful in bear conservation genetics and wildlife forensic.