A pair of two-component regulatory genes ecr A1/A2 in S. coelicolor

Two-component genes are kinds of genetic elements involved in regulation of antibiotic production in Streptomyces coelicolor . DNA microarray analysis revealed that ecr A1/A2, which mapped at distant sites from red lucus and encode respectively the kinase and regulator, expressed coordinately with genes of Red specific biosynthetic pathway. ecr A1 and ecr A2 gene-disruptive mutants were constructed using homogenotisation by reciprocal double crossover. Fermentation data showed that the undecylprodigiosin (Red) level of production was lower than that of wild-type strain. However, the change of the actinorhodin (Act) production level was not significant compared with wild type. Thus, these experiment results confirmed that the two-component system ecr A1/A2 was positive regulatory element for red gene cluster.