Covalent Immobilization of Transglucosidase onto Polymer Beads for Production of Isomaltooligosaccharides

Transglucosidase from Aspergillus niger was immobilized onto Amberzyme oxirane beads and was used for the production of Isomaltooligosaccharides (IMOs) including panose from maltose. The optimum immobilization parameters and, the stability and activity of the immobilized enzyme were determined. Immobilization resulted in 83–85 % immobilization efficiency. The optimum immobilization conditions were 400 mg of Amberzyme oxirane beads with 20 U of transglucosidase at pH 7.5 for 48 h at 25 °C with agitation at 150 rpm. Both immobilized and free enzyme had maximum activity at pH 4 and 60 °C. The results obtained in this study could be used for production of IMOs from maltose with the repeated use of the immobilized enzyme. Graphical Abstract