DETERMINATION OF HALOGENATED PYRENES IN WATER BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY

This paper describes high performance liquid chromatographic (HPLC) determinations of halogenated pyrenes in water by use of LiChrosorb RP-18 column as a stationary phase and acetonitrile/water as a mobile phase. The method consists of the following 3 procedures: extraction of halogenated pyrenes from water samples with benzene, cleaning up by one dimensional dual-band thin layer chromatography, identification and determination of halogenated pyrenes by HPLC. The calibration curves of pyrene (Py), 1-chloropyrene (PyCl), 1-bromopyrene (PyBr), 1,8-,1,6-,1,3-dichloropyrene (PyCl2) and 1,8-dibromopyrene (PyBr2) were linear over the range of 0-2 ng for Py and PyCl, 0-4 ng for each PyCl2, and 0-20 ng for PyBr and 1,8-PyBr2. The minimum detectable amounts of Py and each halogenated pyrenes (s/n = 4) were 0.1 ng for Py, 0.05 ng for PyCl, 0.2 ng for 1,8-PyCl2, 0.25 ng for 1,6-PyCl2, 0.7 ng for 1,3-PyCl2 and 1.0 ng for PyBr and PyBr2. The proposed method has been applied to determine the halogenated compounds in chlorinated-river water spiked with 100-mu-g/l pyrene. The concentrations of halogenated compounds were 0.67-mu-g/l for PyCl, 17.1-mu-g/l for PyBr, 3.87-mu-g/l for PyCl2 and 14.0-mu-g/l for PyBr2.