Disposition and Distribution of the Cytidine Cyanoborane Adduct, [2-14C]-2′-Deoxycytidine-3N-Cyanoborane, in CF1 Mice

2′-Deoxycytidine-3N-cyanoborane (dC-BH2CN) had been found to possess cytotoxic, hypo-lipidaemic, and anti-inflammatory activity; its disposition in mice was investigated in this report. The half-life of dC-BH2CN was found to be 0.3 hours and 2′-deoxycytidine was identified as one metabolite. A second elimination rate-limited metabolite was also formed, but was not identified. dC-BH2CN was taken up in vitro in human BG-9 fibroblasts, rat red blood cells, and murine Tmolt3 cells, and in vivo in Ehrlich ascites carcinoma cells. In the Ehrlich ascites tumour, concentrations of dC-BH2CN were 4- to 8-fold higher than liver and plasma from 2 to 24 hours after administration.