Role of Host and Viral Factors in Negative Antibody Response of Seronegative Occult Hepatitis B Virus Infection

Event Abstract Back to Event Role of Host and Viral Factors in Negative Antibody Response of Seronegative Occult Hepatitis B Virus Infection Ratna S. Wijaya1, 2*, Susan I. Ie1, Yohanna A1, 3, Turyadi Turyadi1, Korri E. El-Khobar1, Alida R. Harahap1, 4 and David H. Muljono1, 5, 6 1 Eijkman Institute for Molecular Biology, Indonesia 2 Faculty of Medicine Pelita Harapan University, Immunology, Indonesia 3 Atma Jaya, Indonesia 4 University of Indonesia, Clinical Pathology, Indonesia 5 Hasanuddin University, Indonesia 6 Sydney Medical School , Australia Background: The lack of antibody response in seronegative occult hepatitis B virus (HBV) infection or OBI can be caused by host and viral factors. The polymorphism of human leukocyte antigen (HLA) class II gene, especially in HLA-DRB1 locus, will determine host antibody immune response whereas mutation in HBV surface (S) gene, which overlaps the HBV polymerase (P) gen, potentially affects the replication ability of HBV. This study aims to investigate the HLA-DRB1 genotypes and the HBV S gene characteristics from OBI seronegative individuals in Ternate. Methods: Host DNA were extracted by salting out method, and further analyzed for HLA-DRB1 alleles together with 9 anti-HBc positive OBI individuals as controls using LABType® SSO Typing Test (One Lambda, USA). HBV S gene of 9 OBI seronegative isolates were detected by PCR, cloned and analyzed for the presence of amino acid variation Results: HLA-DRB1*12 and *15 were the most common alleles. The distribution of these alleles was not statistically different between seropositive and seronegative OBI groups. HBV DNA was found in second round of amplification (<200 IU/mL). From 67 sequenced clones, 60 (89.6%) showed the presence of S gene mutations. Conclusions: Our results suggest that there was no significant difference in HLA-DRB1 allele distribution among OBI seropositive and seronegative groups. The lack of antibody response in OBI seronegative is more likely due to the low level of HBV DNA. This could be caused by mutations in the S gene and its overlapping P gene, which may affect HBV replication. Keywords: Hepatitis B virus, occult infection, seronegative, HLA-DRB1, Mutation Conference: 15th International Congress of Immunology (ICI), Milan, Italy, 22 Aug - 27 Aug, 2013. Presentation Type: Abstract Topic: Host-pathogen interactions Citation: Wijaya RS, Ie SI, A Y, Turyadi T, El-Khobar KE, Harahap AR and Muljono DH (2013). Role of Host and Viral Factors in Negative Antibody Response of Seronegative Occult Hepatitis B Virus Infection. Front. Immunol. Conference Abstract: 15th International Congress of Immunology (ICI). doi: 10.3389/conf.fimmu.2013.02.00626 Copyright: The abstracts in this collection have not been subject to any Frontiers peer review or checks, and are not endorsed by Frontiers. They are made available through the Frontiers publishing platform as a service to conference organizers and presenters. The copyright in the individual abstracts is owned by the author of each abstract or his/her employer unless otherwise stated. Each abstract, as well as the collection of abstracts, are published under a Creative Commons CC-BY 4.0 (attribution) licence (https://creativecommons.org/licenses/by/4.0/) and may thus be reproduced, translated, adapted and be the subject of derivative works provided the authors and Frontiers are attributed. For Frontiers’ terms and conditions please see https://www.frontiersin.org/legal/terms-and-conditions. Received: 12 Jun 2013; Published Online: 22 Aug 2013. * Correspondence: Miss. Ratna S Wijaya, Eijkman Institute for Molecular Biology, Jakarta, Indonesia, Indonesia, ratnasw85@gmail.com Login Required This action requires you to be registered with Frontiers and logged in. To register or login click here. Abstract Info Abstract The Authors in Frontiers Ratna S Wijaya Susan I Ie Yohanna A Turyadi Turyadi Korri E El-Khobar Alida R Harahap David H Muljono Google Ratna S Wijaya Susan I Ie Yohanna A Turyadi Turyadi Korri E El-Khobar Alida R Harahap David H Muljono Google Scholar Ratna S Wijaya Susan I Ie Yohanna A Turyadi Turyadi Korri E El-Khobar Alida R Harahap David H Muljono PubMed Ratna S Wijaya Susan I Ie Yohanna A Turyadi Turyadi Korri E El-Khobar Alida R Harahap David H Muljono Related Article in Frontiers Google Scholar PubMed Abstract Close Back to top Javascript is disabled. Please enable Javascript in your browser settings in order to see all the content on this page.