The effect of volume of interest definition on quantification of lymph node immune response to a monkeypox virus infection assessed by 18 F-FDG-PET

Background 2-deoxy-2-[ 18 F]fluoro-D-glucose-positron emission tomography ( 18 F-FDG-PET) is applied in the clinic for infection assessment and is under consideration for investigating the inflammatory/immune response in lymphoid tissue in animal models of viral infection. Assessing changes in 18 F-FDG uptake of lymph nodes (LNs), primary lymphoid tissues targeted during viral infection, requires suitable methods for image analysis. Similar to tumor evaluation, reliable quantitation of the LN function via multiple 18 F-FDG-PET sessions will depend how the volume of interest is defined. Volume of interest definition has a direct effect on statistical outcome. The current study objective is to compare for the first time agreement between conventional and modified VOI metrics to determine which method(s) provide(s) reproducible standardized uptake values (SUVs) for 18 F-FDG uptake in the LN of rhesus macaques. Methods Multiple 18 F-FDG-PET images of LNs in macaques were acquired prior to and after monkeypox virus intravenous inoculation. We compared five image analysis approaches, SUV max , SUV mean , SUV threshold , modified SUV threshold , and SUV fixed volume , to investigate the impact of these approaches on quantification of the changes in LN metabolic activity denoting the immune response during viral infection progression. Results The lowest data repeatability was observed with SUV max . The best correspondence was between SUV fixed volume and conventional and modified SUV threshold . A statistically significant difference in the LN 18 F-FDG uptake between surviving and moribund animals was shown using modified SUV threshold and SUV fixed volume (adjusted p = 0.0037 and p = 0.0001, respectively). Conclusions Quantification of the LN 18 F-FDG uptake is highly sensitive to the method applied for PET image analysis. SUV fixed volume and modified SUV threshold demonstrate better reproducibility for SUV estimates than SUV max , SUV mean , and SUV threshold . SUV fixed volume and modified SUV threshold are capable of distinguishing between groups with different disease outcomes. Therefore, these methods are the preferred approaches for evaluating the LN function during viral infection by 18 F-FDG-PET. Validation of multiple approaches is necessary to choose a suitable method to monitor changes in LN metabolic activity during progression of viral infection.