Thin Film Biosensor Based on Organic-inorganic Hybrid System

Integration of organic and inorganic material is a promising method for obtaining unusual functional material. Recently, we developed a new fabrication method through a combination of Langmuir-Blodgett (LB) and self-assembled monolayers (SAMs) techniques. Using this method, an enzyme of glucose oxidase was chemically bonded with –COOH terminal group of SAM. After that, Prussian Blue nano-clusters were electrostatically immobilized in the octadecyltrimethylammonium LB film. The amperometric glucose biosensor prepared with this method showed a stable linear relationship and a high stability of the sensor performance depending on the glucose concentration at a low potential range. It was found that the current density was affected by the molecular aliphatic chain length in the SAMs layer.