The comparative study on antioxidant capacity of Keshan disease and dilated cardiomyopathy patients

Objective : To compare the serum levels of selenium, selenoprotein and malondialdehyde about Keshan disease (KSD) and dilated cardiomyopathy (DCM) patients and to investigate the relationship between the antioxidant capacity and myocardial injury. Method s: 26 KSD patients, 18 DCM patients, 24 healthy people in KSD areas and 22 healthy people in non-KSD areas were selected by all-round physical examination and electrocardiography. Blood samples were collected by informing consent and volunteering. The serum selenium was determined by using 2,3 - two amino-naphthalene fluorescence method. The serum selenoprotein and malondialdehyde were measured by using biotin double-antibody sandwich method of enzyme linked immunosorbent assay (ELISA) (according to kit instructions). Result s: There were no statistical differences about the distribution of the gender and age in every subjects’ groups ( P ＞0.05). The levels of serum selenium and selenoprotein in Keshan disease group and dilated cardiomyopathy group were significantly lower than those of healthy group whether in KSD areas or in non- KSD areas , and the serum malondialdehyde were higher than those in healthy controls. There were no statistical significance about the concentrations of serum selenium, selenoprotein and malondialdehyde between Keshan disease group and dilated cardiomyopathy group, KSD areas’ control group and non- KSD areas’ control group. Conclusion : The myocardial injury of KSD patients and dilated cardiomyopathy patients may be associated with the decrease of antioxidant ability because of the lower selenium levels.