A Highly Selective And Sensitive Fluorescent Chemosensor For Detection Of Cn-, So32- And Fe3+ Based On Aggregation-Induced Emission

A novel fluorescence chemosensor 1 with aggregation-induced emission was designed and synthesized through a nucleophilic addition reaction between cyanide and triphenylamine. It exhibited remarkable selectivity and high sensitivity and was able to detect Fe3+, CN- and SO32- in almost pure aqueous solution with low detection limits of 1.44 mu M, 9.88 nM and 0.107 mu M, respectively. Job's plot and H-1 NMR data showed that the binding stoichiometry of 1 with Fe3+, CN- or SO32- was 1:1. Further observations of H-1 NMR titration suggested that a coordination bond was formed between two cyano of 1 and Fe3+ which resulted in fluorescence quenching of 1 after detection of Fe3+, whereas the nucleophilic addition of cyanide or sulfite to the vinyl group was responsible for the fluorescent quenching of CN- or SO32- toward 1. The biological applications of 1 were also evaluated and it was found to exhibit low cytotoxicity and membrane permeability. In addition, 1 could also be made into test strips to detect Fe3+ and CN- and was employed as a sensor for detection Fe3+ in living cells.