13-week dietary study and in vitro and in vivo genotoxicity studies of a structuring fat produced through a microalgal fermentation process

•The dietary safety of a unique microalgal structuring fat produced by a heterotrophic fermentation process was assessed.•The algal fat was non-mutagenic in Salmonella typhimurium and Escherichia coli tester strains at up to 5000μg/plate.•The algal fat was non-clastogenic in mouse bone marrow after a single oral dose at 2000mg/kg bw.•Dietary NOAEL of the algal structuring fat for rats in a 13-week study was 100000ppm, the highest concentration tested.•Based on consumption, the oral NOAEL for the algal structuring fat in rats was 5299 (male) and 6313.8 (female) mg/kg bw/day.