Effect of follicle-stimulating hormone and testosterone on colony formation of bovine spermatogonial stem cell
Comparative Clinical Pathology(2013)
摘要
The complex process of spermatogenesis is regulated by various factors. In this study, the in vitro effects of follicle-stimulating hormone and testosterone on spermatogonial cell colony formation were investigated. Sertoli and spermatogonial cells were isolated from 3- to 5-month-old calves. Co-cultured Sertoli and spermatogonial cells were treated with follicle-stimulating hormone and testosterone in treatment groups before colony assay. Results indicated that follicle-stimulating hormone is the best factor for in vitro colonization of spermatogonial cells. In conclusion, using follicle-stimulating hormone provided proper bovine spermatogonial stem cell culture medium for in vitro study of these cells.
更多查看译文
关键词
Co-culture,Bovine,FSH,Testosterone
AI 理解论文
溯源树
样例
生成溯源树,研究论文发展脉络
Chat Paper
正在生成论文摘要