Real-Time Image Processor For Detection Of Rare Cells And Particles In Flow At 37 Mhz Line Scans Per Second
IMAGING, MANIPULATION, AND ANALYSIS OF BIOMOLECULES, CELLS, AND TISSUES XI(2013)
摘要
We describe a real-time image processor that has enabled a new automated flow through microscope to screen cells in flow at 100,000 cells/s and a record false positive rate of one in a million. This unit is integrated with an ultrafast optical imaging modality known as serial time-encoded amplified microscopy (STEAM) for blur-free imaging of particles in high-speed flow. We show real-time image-based identification and screening of budding yeast cells and rare breast cancer cells in blood. The system generates E-slides (an electronic version of glass slides) on which particles of interest are digitally analyzed.
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关键词
Medical diagnostics, High-throughput screening, Instrumentation, Microfluidics, Photonics, Real-time image processing, Cancer cell detection, High-speed camera
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