Development and validation of a simple and economical spectrofluorimetric method for estimation of quinine in pharmaceutical dosage forms

A new simple, sensitive, precise, economic and “gr een” spectrofluorimetric method for the determination of quinine both as a bulk drug and in tablet formulations was developed and validated us ing water as solvent. At a predetermined excitation wav elength (330 nm) and emission wavelength (380 nm), it was proved linear in the concentration range of 5050 ng/mL, exhibited good correlation coefficient ( R= 0.999) and excellent mean recovery (97.5-103%). The results of the recovery studies showed that the me thod was not affected by the presence of common excipien ts. The method was applied for the analysis of the drug in the pure, tablet and injectable forms. The method w as validated for precision, accuracy and recovery s tudies. Limit of Detection and Limit of Quantification for quinine were found to be 16.6 ng/mL and 19.8 ng/mL respectively. The method has been successfully appl ied for the analysis of marketed formulations avail able in Senegal. © 2013 International Formulae Group. All rights res erved.